![]() Please feel free to add information about a computation tool for synthetic biology (CADs, simulators, databases, lab managements systems, automation software, anything) to the list. This list was seeded by the participants of the 2008 workshop on Standards and Specifications in Synthetic Biology. 18 Ribosome Binding Site (RBS) Calculator.2017.Comprehensive analysis of amino acid sequence diversity at the F protein cleavage site of Newcastle disease virus in fusogenic activity. Complete Genome Sequence of a Vaccinal Newcastle Disease Virus Strain Isolated from an Owl (Rhinoptynx clamator). ![]() Rescue of Newcastle disease virus from cloned cDNA: evidence that cleavability of the fusion protein is a major determinant for virulence. Archives of Virology 162:1-12ġ5 Peeters,B., P. Rescue of recombinant Newcastle disease virus: current cloning strategies and RNA polymerase provision systems. Characterization of Malaysian velogenic NDV strain AF2240-I genomic sequence: a comparative study.Virus Genes 46:431-440.ġ4 Molouki, A and B. Acta Trop 183:126-133.ġ3 Kavitha Murulitharan, Khadijah Yusoff., Abdul Rahman Omar and Aidin Molouki. Newcastle disease virus strain AF2240 as an oncolytic virus: A review. Identification of novel Newcastle disease virus sub-genotype VII-(j) based on the fusion protein. Clone Purification, Characterization and Standardization of LaSota Strain for Developing a Live Vaccine against Newcastle Disease Virus. Veterinary Microbiology 160:17-20.ġ0.Ebrahimi, M.M., Shahsavandi. 2012 Newcastle disease virus outbreaks: vaccine mismatch or inadequate application. Journal of Clinical Microbiology 50:378-87.ĩ Dortmans, J. Complete Genome and Clinicopathological Characterization of a Virulent Newcastle Disease Virus Isolate from South America. Journal of General Virology 80:131-136.Ĩ. Complete nucleotide sequence of Newcastle disease virus: evidence for the existence of anew genus within the subfamily Paramyxovirinae. Evaluation of imidazole and its derivative against Newcastle disease virus Infection in chicken: A drug repurposing approach. Emerging Infectious Diseases 19: 93-98.Ħ. The Foundations of Virology: Discoverers and Discoveries, Inventors and Inventions, Developers and Technologies. The long view: a selective review of 40 years of Newcastle disease research. Detection and differentiation of Newcastle disease virus (avian paramyxovirus type 1). Complete genome sequence of a velogenic Newcastle disease virus isolated in Mexico.Virus Genes 45:304-310.ģ. Research in Veterinary Science 93:151-154.Ģ. Molecular characterization of a recent Newcastle disease virus outbreak in Jordan. Location of each start (S) and ending (E) gene signals as well as all ORF start and termination codons were determined.ġ. Sequence of the whole 15186 bp genome was determined by assembling sequences using MEGA 5software. After confirmation, the inserts were sequenced using Sanger sequencing with pJEt specific primers as well as primers designed to bind to middle regions of each fragment. Successful cloning of each insert was confirmed using restriction enzyme BglII that digests areas flanking the insert in pJET. PCR was run using 7 sets of primers covering the whole cDNA.The PCR products were all gel extracted and ligated to pJET1.2 vector by 3-1 ratios. ![]() RNA was extracted and cDNA spanning the whole genome was synthesized using two GSP primers. The virus was purified using sucrose-gradient. plaque purified NDV strain clone IR12 produced by Razi Institute was propagated in 10-day-old SPF emberyonated chicken eggs, then allantoic fluid was harvested. Despite vaccination with both live attenuated and inactivated NDV strains to immunize birds, ND is still a serious problem in poultry industry especially in developing countries like Iran. Newcastle disease (ND) is an important viral disease of poultry causing severe economic losses and drop in egg production in broiler and layers flocks.
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